The research focus of the Core Facility Hard Tissue and Biomaterial Research, Karl Donath Laboratory is the morphological, histological and histomorphometric assessment of preclinical and clinical studies for research on bone substitute materials, biomaterials, implants and diseases of the oral cavity, as well as basic osteological research.
Our Research Focus:
- Bone Regeneration: We are evaluating innovative treatment methods to enhance bone healing.
- Optimization of Bone Augmentation Materials: Modifications to these materials are being examined for their impact on bone growth, particularly for future implant placement.
- Improving Osseointegration of Implants: Research is being conducted into how the integration and long-term load-bearing capacity of dental implants in the jawbone can be optimized.
- Genetic Mutations: We conduct radiologic and histologic studies to biologically and clinically understand the changes in teeth and bone caused by genetic mutations in transgenic animals.
- Evolutionary and Forensic Osteology and Anthropology - Infantile Cranial Development: Using high-resolution micro-computed tomography (microCT), we study the growth and development of the infant skull.
Applied Methods
- Production of undecalcified histological thin sections: Highly accurate histological thin-ground sections are produced using the sawing-grinding technique established by Karl Donath, allowing detailed, standardised and reproducible analysis.
- Virtual histology: Using special microscope systems, we produce image files at any magnification, which can be easily made available digitally for virtual microscopy.
- MicroCT scans and 3D reconstructions: We use high-resolution microCT scans to generate 3D representations of target structures and subsequent analysis
Dr. Stefan Tangl
Head of the Core Facility Hard Tissue and Biomaterial Research, Karl Donath Laboratory
E: stefan.tangl@meduniwien.ac.at
ORCID: 0000-0002-9023-4745
Mag. Carina Kampleitner
Deputy Head of Core Facility Hard Tissue and Biomaterial Research, Karl Donath Laboratory
E: carina.kampleitner@meduniwien.ac.at
ORCID: 0000-0003-3072-5072
Mag. Dr. Karoline Reich
Research Associate
Assistant in the Clinical Division of Prosthodontics
E: karoline.reich@meduniwien.ac.at
ORCID: 0000-0002-8835-369X
Toni Dobsak
Research Associate – 3D Visualisation, Microscopy, Histomorphometry
E: anton.dobsak@meduniwien.ac.at
ORCID: 0000-0003-0152-9419
Patrick Heimel, MSc.
Research Associate - BioImaging, Histomorphometry
E: patrick.heimel@trauma.lbg.ac.at
ORCID: 0000-0002-2499-325X
Stefan Lettner
Anna Reisinger, BSc
Laboratory Management, Laboratory Techniques
Karol Ali Apaza Alccayhuaman, DDS, MSc
Research Guests:
- Eloá Luvizuto (Brazil)
- Fernando M.M. Guzón (Spain)
- Gabriel Mulinari dos Santos (Brazil)
- Gino Inverso (USA)
- Lara Cervantes (Brazil)
- Marta Kisiel (Sweden)
- Mei Lu (USA)
- Miggue Wang (China)
- Reiko Kobatake (Japan)
- Roberta Michels (Brazil)
- Siddhart Shanbhag (Norway)
- Tereza Kubíková (Czech Republic)
- Zhang Yu (China)
- Zhou Wenjuan (China)
Former Employees:
- Amin Rezakhani
- Bernadette Luza
- Bernd Bernegger
- Christian D. Huber
- Dieter Busenlechner
- Franziska Kirchweger
- Kevin Doppelmayer
- Michael Sandholzer
- Thomas Schwaha
- Uwe Yacine Schwarze
Doctoral Students:
- Alexandra Meinl
- Dieter Busenlechner
- Karoline Reich
- Ludwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, Vienna, Austria, Assoc. Prof. Dr. Johannes Grillari, a.o. Univ. Prof. Dr. Heinz Redl
- Araçatuba Dental School, Universidade Estadual Paulista "Júlio de Mesquita Filho", Araçatuba, Brazil
- Center for Education and Research on Dental Implants (CEPID), Department of Dentistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil, Dr. Prof. Cesar Augusto Magalhães Benfatti
- Center for Forensic Medicine, Unit of Forensic Anthropology, Medical University of Vienna, Vienna, Austria, Assoc.-Prof. Ing. Mag. Dr. rer. nat. Fabian Kanz
- Department für Zahnärztliche Chirurgie und Röntgenologie, Univ. Klinik für Zahn-, Mund- und Kieferheilkunde, Medizinische Universität Graz, Graz, Austria, a.o. Univ. Prof. DDr. Norbert Jakse, Assoc. Prof. Priv. Doz. DDr. Michael Payer
- Department of Anthropology, Natural History Museum Vienna, Vienna, Austria, HR ao. Univ.- Prof. Dr. Maria Teschler-Nicola
- Department of Clinical Dentistry, Tissue Engineering Group, Faculty of Medicine, University of Bergen, Bergen, Norway, Siddharth Shanbhag, PhD
- Department of Evolutionary Anthropology, University of Vienna, Vienna, Austria, Univ.-Prof. Dr. Gerhard Weber
- Department of Oral and Maxillofacial Surgery, Dental School, University of Athens, Athen, Greece, Dr. Efstathia Tsetsenekou
- Department of Orthopaedic Surgery, Karl Chiari Lab for Orthopaedic Biology, Medical University of Vienna, Vienna General Hospital, Vienna, Austria, Assoc. Prof. Priv. Doz. Mag. Dr. Stefan Tögel
- Department of Orthopaedic Surgery, Medical University of Vienna, Vienna General Hospital, Vienna, Austria, Univ. Prof. Dr. Reinhard Windhager
- Fakultät für Technik und Umweltwissenschaften, Werkstoff- und Produktionstechnik, MicroCT, FH Oberösterreich Campus Wels, Wels, Austria, DI Salaberger Dietmar
- French Institute of Health and Medical Research, INSERM, Unit ToNIC, UMR 1214, Toulouse, France, Pierre Layrolle, PhD
- Institute of Physics and Materials Science, BOKU University, Vienna, Austria, Univ.-Prof. i.R. Dr. Stefanie Tschegg
- Klinische Abteilung für allgemeine Kinder- und Jugendchirurgie, Univ.-Klinik für Kinder- und Jugendchirurgie, Medizinische Universität Graz, Graz, Austria, Ass. Prof. Dr. Annelie-Martina Weinberg, Priv.-Doz. Dr. Christoph Castellani
- Laura Bassi Centres of Expertise: BRIC - BioResorbable Implants for Children
- Pathological-anatomical Museum, Narrenturm, Vienna, Austria
- Prothetik-, Biomechanik- und Biomaterialforschung, Paracelsus Medizinische Privatuniversität, Salzburg, Austria, DDr. Peter Schuller Götzburg
- Universidade Estadual Paulista (UNESP), Sao Paulo, Brazil, Dr. Eloá Luvizuto
- Humbert P, Kampleitner C, De Lima J, Brennan MÁ, Lodoso-Torrecilla I, Sadowska JM, Blanchard F, Canal C, Ginebra MP, Hoffmann O, Layrolle P. Phase composition of calcium phosphate materials affects bone formation by modulating osteoclastogenesis. Acta Biomater. 2024 Mar 1;176:417-431. doi: 10.1016/j.actbio.2024.01.022. Epub 2024 Jan 23. PMID: 38272200.
- Apaza Alccayhuaman KA, Heimel P, Tangl S, Lettner S, Kampleitner C, Panahipour L, Kuchler U, Gruber R. Active and Passive Mineralization of Bio-Gide® Membranes in Rat Calvaria Defects. J Funct Biomater. 2024 Feb 21;15(3):54. doi: 10.3390/jfb15030054. PMID: 38535247; PMCID: PMC10970795.
- Feher B, Kampleitner C, Heimel P, Tangl S, Helms JA, Kuchler U, Gruber R. The effect of osteocyte-derived RANKL on bone graft remodeling: An in vivo experimental study. Clin Oral Implants Res. 2023 Dec;34(12):1417-1427. doi: 10.1111/clr.14187. Epub 2023 Oct 4. PMID: 37792417.
- Apaza Alccayhuaman KA, Heimel P, Lee JS, Tangl S, Kuchler U, Marchesan J, Panahipour L, Lettner S, Matalová E, Gruber R. FasL is a catabolic factor in alveolar bone homeostasis. J Clin Periodontol. 2023 Mar;50(3):396-405. doi: 10.1111/jcpe.13750. Epub 2022 Nov 25. PMID: 36384160; PMCID: PMC10946845.
- Shanbhag S, Kampleitner C, Al-Sharabi N, Mohamed-Ahmed S, Apaza Alccayhuaman KA, Heimel P, Tangl S, Beinlich A, Rana N, Sanz M, Kristoffersen EK, Mustafa K, Gruber R. Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects. Cells. 2023 Feb 28;12(5):767. doi: 10.3390/cells12050767. PMID: 36899904; PMCID: PMC10001262.
- Reich KM, Beck F, Heimel P, Lettner S, Redl H, Ulm C, Tangl S. Bone Graft Packing and Its Association with Bone Regeneration in Maxillary Sinus Floor Augmentations: Histomorphometric Analysis of Human Biopsies. Biology (Basel). 2022 Sep 30;11(10):1431. doi: 10.3390/biology11101431. PMID: 36290335; PMCID: PMC9598793.
- Shanbhag S, Kampleitner C, Mohamed-Ahmed S, Yassin MA, Dongre H, Costea DE, Tangl S, Stavropoulos A, Bolstad AI, Suliman S, Mustafa K. Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs. Front Bioeng Biotechnol. 2021 Nov 30;9:783468. doi: 10.3389/fbioe.2021.783468. Erratum in: Front Bioeng Biotechnol. 2022 Jan 04;9:831669. doi: 10.3389/fbioe.2021.831669. PMID: 34917602; PMCID: PMC8670384.
- Shanbhag S, Suliman S, Mohamed-Ahmed S, Kampleitner C, Hassan MN, Heimel P, Dobsak T, Tangl S, Bolstad AI, Mustafa K. Bone regeneration in rat calvarial defects using dissociated or spheroid mesenchymal stromal cells in scaffold-hydrogel constructs. Stem Cell Res Ther. 2021 Nov 14;12(1):575. doi: 10.1186/s13287-021-02642-w. PMID: 34776000; PMCID: PMC8591809.
- Strauss FJ, Kuchler U, Kobatake R, Heimel P, Tangl S, Gruber R. Acid bone lysates reduce bone regeneration in rat calvaria defects. J Biomed Mater Res A. 2021 May;109(5):659-665. doi: 10.1002/jbm.a.37050. Epub 2020 Jul 10. PMID: 32608132.
- Beck F, Reich KM, Lettner S, Heimel P, Tangl S, Redl H, Ulm C. The vertical course of bone regeneration in maxillary sinus floor augmentations: A histomorphometric analysis of human biopsies. J Periodontol. 2021 Feb;92(2):263-272. doi: 10.1002/JPER.19-0656. Epub 2020 Aug 19. PMID: 32715479.
Dr. Stefan Tangl
Head of the Core Facility Hard Tissue and Biomaterial Research, Karl Donath Laboratory
T: +43 (0)1 40070-2628
E: stefan.tangl@meduniwien.ac.at
ORCID: 0000-0002-9023-4745
University Clinic of Dentistry
Medical University of Vienna
Sensengasse 2a
1090 Vienna